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  1. Otaki M., Hirane N., Natsume-Kitatani Y., Nogami Itoh M., Shindo M., Kurebayashi Y., Nishimura S-I.
    Mouse tissue glycome atlas 2022 highlights inter-organ variation in major N-glycan profiles.
    Sci. Rep. 12, 17804 (2022)

  2. Shiratori K., Yokoi Y., Wakui H., Hirane N., Otaki M., Hinou H., Yoneyama T., Hatakeyama T., Kimura S., Ohyama C., Nishimura S.-I.
    Selective reaction monitoring approach using structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics.
    RSC Adv. 12, 21385-21393 (2022)

  3. Takei D., Harada K., Nouso K., MiyaharaK., Dohi C., Matsushita H., Kinugasa H., Hiraoka S., Nishimura S-I., Okada H.
    Clinical utility of a serum glycome analysis in patients with colorectal cancer.
    J. Gastroen. Hepatol. 37, 727-733 (2022)

  4. Takahashi H., Kamiyama T., Hirane N., Kobayashi N., Aiyama T., Nagatsu A., Shimada S., Orimo T., Kakisaka T., Fukai M., Yokoo H., Kamachi H., Nishimura S-I., Taketomi A.
    Analysis of the correlation between alterations in N?glycans and invasiveness in liver cancer cell lines.
    Oncol. Rep. 44, 2757-2769 (2020)

  5. Pablo A. Guillen-Poza, Elena Matilde Sanchez-Fernandez, Gerard Artigas, Jose Manuel Garcia Fernandez, Hinou H., Carmen Ortiz Mellet, Nishimura S-I., Fayna Garcia-Martin.
    Amplified detection of breast cancer autoantibodies using MUC1-based Tn antigen mimics.
    J. Med. Chem. 63, 8524-8533 (2020)

  6. Hayakawa A., Matsushita T., Yokoi Y., Wakui H., Fayna Garcia-Martin, Hinou H., Matsuoka K., Nouso H., Kamiyama T., Taketomi A, Nishimura S-I.
    Impaired O-Glycosylation at Consecutive Threonine TTX Motifs in Mucins Generates Conformationally Restricted Cancer Neoepitopes.
    Biochemistry 59, 1221-1241 (2020)

  7. Soma O., Hatakeyama S., Yoneyama T., Saito M., Sasaki H., Tobisawa Y., Noro D., Suzuki Y., Tanaka M., Nishimura S-I., Harada H., Ishida H., Tanabe K., Satoh S., Ohyama C.
    Serum N-glycan profiling can predict biopsy-proven graft rejection after living kidney transplantation.
    Clin. Exp. Nephrol. 24, 174-184 (2020)

  8. Matsumoto T., Hatakeyama S., Yoneyama T., Tobisawa Y., Ishibashi Y., Yamamoto H., Yoneyama T., Hashimoto Y., Ito H., Nishimura S-I., Ohyama C.
    Serum N-glycan profiling is a potential biomarker for castration-resistant prostate cancer.
    Sci. Rep. 9, 168761 (2019)

  9. Gebrehiwot Abrha G., Seifu Melka D., Mamo Kassaye Y., Gemechu T., Lako W., Hinou H., Nishimura S-I.
    Exploring serum and Immunoglobulin G N-glycome as diagnostic biomarkers for early detection of breast cancer in Ethiopian women.
    BMC Cancer 19, 588 (2019)

  10. Gebrehiwot Abrha G., Seifu Melka D., Mamo Kassaye Y., Rehan F.I., Rangappa S., Hinou H., Kamiyama T., Nishimura S-I.
    Healthy human serum N-glycan profiling reveals the influence of ethnic variation on the identified cancer-relevant glycan biomarkers.
    PLOS ONE 13, e0209515 (2018)

  11. Ishihara S., Aoki K., Mizutani T., Amano M., Nishimura S-I., Haga H.
    Glycosphingolipid GM2 induces invasiveness in irradiation-tolerant lung cancer cells.
    Cell Struct. Funct. 43, 177-185 (2018)

  12. Yogesh K.V., Kamiyama T, Ohyama C., Yoneyama T., Nouso K., Kimura S., Hinou H., Nishimura S-I.
    Synthetic glycopeptides as a designated standard in focused glycoproteomics to discover serum cancer biomarkers.
    MedChemComm 9, 1351-1358 (2018)

  13. Tanaka T., Yoneyama T., Noro D., Imanishi K., Kojima Y., Hatakeyama S., Tobisawa Y., Mori K., Yamamoto H., Imai A., Yoneyama T., Hashimoto Y., Koie T., Tanaka M., Nishimura S-I., Kurauchi S., Takahashi I., Ohyama C.
    Aberrant N-Glycosylation Profile of Serum Immunoglobulins is a Diagnostic Biomarker of Urothelial Carcinomas.
    Int. J. Mol. Sci. 18, 2632 (2017)

  14. Noro D., Yoneyama T., Hatakeyama S., Tobisawa Y., Mori K., Hashimoto Y., Koie T., Tanaka M., Nishimura S-I., Sasaki H., Saito M., Harada H., Chikaraishi T., Ishida H., Tanabe K., Satoh S., and Ohyama C.
    Serum Aberrant N-Glycan Profile as a Marker Associated with Early Antibody-Mediated Rejection in Patients Receiving a Living Donor Kidney Transplant.
    Int. J. Mol. Sci. 18, 1731 (2017)

  15. Sato R., Tsuchiya K., Matsuzaki H., Takei N., Itoh H., Kanayama N., Suda T., Watanabe H., Ohashi T., Tanaka M., Nishimura S-I., Maekawa M.
    Fetal environment and glycosylation status in neonatal cord blood: a comprehensive mass spectrometry-based glycosylation analysis.
    Medicine 94, e3219 (2016)

  16. Gizaw S., Ohashi T., Tanaka M., Hinou H., Nishimura S-I.
    Glycoblotting method allows for rapid and efficient glycome profiling of human Alzheimer’s disease brain, serum and cerebrospinal fluid towards potential biomarker discovery.
    BBA-GenSubjects 1860, 1716-1727 (2016)

  17. Inafuku S., Noda K., Amano M., Nishimura S-I., Ishida S.
    Increase of Sialylated N-glycans in Eyes with Neovascular Glaucoma Secondary to Proliferative Diabetic Retinopathy.
    Curr. Eye Res. 41, 721-724 (2016)

  18. Tomoda T., Nouso K., Kato H., Miyahara K., Dohi C., Morimoto Y., Kinugasa H., Akimoto Y., Matsumoto K., Yamamoto N., Noma Y., Horiguchi S., Tsutsumi K., Amano M., Nishimura S-I., Yamamoto K.
    Alteration of serum N-glycan profile in patients with autoimmune pancreatitis.
    Pancreatology 16, 44-51 (2015)

  19. Akimoto Y., Nouso K., Kato H., Miyahara K., Dohi C., Morimoto Y., Kinugasa H., Tomoda T., Yamamoto N., Tsutsumi K., Kuwaki K., Onishi H., Ikeda F., Nakamura S., Shiraha H., Takaki A., Okada H., Amano M., Nishimura S-I., Yamamoto K.
    Serum N-glycan profiles in patients with intraductal papillary mucinous neoplasms of the pancreas.
    Pancreatology 15, 432-438 (2015)

  20. Inafuku S., Noda K., Amano M., Murata M., Saito W., Ohashi T., Kanda A., Nishimura S-I., Ishida S.
    Alteration of N-glycan profiles in Diabetic Retinopathy.
    Invest. Ophthalmol. Vis. Sci. 56, 5316-5322 (2015)

  21. Gizaw S., Koda T., Amano M., Kamimura K., Ohashi T., Hinou H., Nishimura S-I.
    A comprehensive glycome profiling of Huntington's disease transgenic mice.
    BBA-GenSubjects 1850, 1704-1718 (2015)

  22. Miyahara K., Nouso K., Morimoto Y., Kinugasa H., Kato H., Yamamoto N., Tsutsumi K., Kuwaki., K., Onishi H., Ikeda F., Nakamura S., Shiraha H., Takaki A., Nakahara T., Miura Y., Asada H., Amano M., Nishimura S-I., Yamamoto K.
    Prognostic Value of Altered N-Glycosylation of Circulating Glycoproteins in Patients With Unresectable Pancreatic Cancer Treated With Gemcitabine.
    Pancreas 44, 551-556 (2015)

  23. Miyahara K., Nouso K., Dohi C., Morimoto Y., Kinugasa H., WADA N., Takeuchi Y., Kuwaki K., Ohnishi H., Ikeda F., Miyake Y., Nakamura S., Shiraha H., Takaki A., Amano M., Nishimura S-I., and Yamamoto K.
    Alteration of N-glycan profiles in patients with chronic hepatitis and hepatocellular carcinoma.
    Hepatol. Res. 45, 986-993 (2015)

  24. Yamasaki Y., Nouso K., Miyahara K., Wada N., Dohi C., Morimoto Y., Takeuchi Y., Yasunaka T., Kuwaki K., Onishi H., Ikeda F., Miyake Y., Nakamura S., Shiraha H., Takaki A., Amano M., Nishimura S-I., and Yamamoto K.
    Use of noninvasive serum glycan markers to distinguish nonalcoholic steatohepatitis from simple steatosis.
    J. Gastroen. Hepatol. 30, 528-534 (2015)

  25. Terashima M., Amano M., Onodera T., Nishimura S-I., and Iwasaki N.
    Quantitative glycomics monitoring of induced pluripotent and embryonic stem cells during neuronal differentiation.
    Stem. Cell Res 13, 454-464 (2014)

  26. Ishibashi Y., Tobisawa Y., Hatakeyama S., Ohashi T., Tanaka M., Narita S., Koike T., Habuchi T., Nishimura S-I., Ohyama C., Yoneyama T.
    Serum tri- and tetra-antennary N-glycan is a potential predictive biomarker for castration-resistant prostate cancer.
    The prostate 74, 1521-1529 (2014)

  27. Inafuku S., Noda K., Amano M., Ohashi T., Yoshizawa C., Saito W., Kanda A., Nishimura S-I., Ishida S.
    A comparison of N-glycan profiles in human plasma and vitreous fluid
    Graefes Arch. Clin. Exp. Ophthalmol. 252, 1235-1243 (2014)

  28. Hatakeyama S., Amano M., Tobisawa Y., Yoneyama T., Tsuchiya N., Habuchi T., Nishimura S-I., Ohyama C.
    Discovery of novel differentiation markers in the early stage of chondrogenesis by glycoform-focused reverse proteomics and genomics.
    BBA-GenSubjects 1840, 645-655 (2014)

  29. Ishihara T., Kakiya K., Takahashi K., Miwa H., Rokushima M., Yoshinaga T., Tanaka T., Togame T., Takemoto H., Amano M., Iwasaki N.,Minami A., Nishimura S-I.
    Serum N-glycan alteration associated with renal cell carcinoma detected by high-throughput glycan analysis.
    J. Urol. 191, 805-813 (2014)

  30. Miyahara K., Nouso K., Miyake Y., Nakamura S., Obi S., Amano M., Hirose K., Nishimura S-I., Yamamoto K.
    Serum glycan as a prognostic marker in patients with advanced hepatocellular carcinoma treated with sorafenib.
    Hepatology, 59, 355-356 (2014)

  31. Hatakeyama S., Amano M., Tobisawa Y., Yoneyama T., Tsushima M., Hirose K., Yoneyama T., Hashimoto Y., Koie T., Saitoh H., Yamaya K., Funyu T., Nisimura S-I., and Ohyama C.
    Serum N-glycan profiling predicts prognosis in patients undergoing hemodialysis.
    Sci. World J. 2013, ID268407 (2013)

  32. Takeuchi M., Amano M., Kitamura H., Tsukamoto T., Masumori N., Hirose K., Ohashi T and Nishimura S-I.
    N- and O-glycome analysis of serum and urine from bladder cancer patients using a high-throughput glycoblotting method.
    Journal of Glycomics & Lipidomics 3, 108 (2013)

  33. Miyahara K., Nouso K., Saito S., Hiraoka S., Harada K., Takahashi S., Morimoto Y., Kobayashi S., Ikeda F., Miyake Y., Shiraha H., Takaki A., Amano, M. Nishimura S.-I, Yamamoto K.
    Serum glycan markers for evaluation of disease activity and prediction of clinical course in patients with ulcerative colitis.
    PLOS ONE 8, e74861 (2013)

  34. Nouso K., Amano M., Miyahara K., Ito M. Y., Miyahara K., Morimoto Y., Kato H., Tsutsumi K., Tomoda T., Yamamoto N., Nakamura S., Kobayashi S., Kuwaki K., Hagihara H., Onishi H., Miyake Y., Ikeda F., Shiraha H., Takaki A., Nakahara T., Nishimura S-I., Yamamoto K.
    Clinical utility of high-throughput glycome analysis in patients with pancreatic cancer.
    J. Gastroenterology 48, 1171-1179 (2013)

  35. Kamiyama T., Yokoo H., Furukawa J., Kurogochi M., Togashi T., Miura N., Nakanishi K., Kamachi H., Kakisaka T., Tsuruga Y., Taketomi A., Nishimura S-I., Todo S.
    Identification of novel serum biomarkers of hepatocellular carcinoma using glycomic analysis.
    Hepatology 57, 2314-2325 (2013)

  36. Urita A., Matsuhashi T., Onodera T., Nakagawa H., Hato M., Amano M., Seito N., Minami A., and Nishimura S-I.
    Alterations of High-mannose Type N-Glycosylation in Human and Mouse Ostesarthritis Cartilage.
    Arthritis & Rheumatism 63, 3428-3438 (2011)

  37. Irie T., Majima T., Sawaguchi N., Funakoshi T., Nishimura S.-I., and Minami A.
    “Biomechanical and histologic evaluation of tissue enjinnered ligaments using chitosan and hyaluronan hybrid polymer fibers: A rabbit medial collateral ligament reconstruction model.
    J. Biomed. Mater. Res. Part A. 97A, 111-117 (2011)

  38. Miura Y, Kato K, Takegawa Y, Kurogochi M, Furukawa J, Shinohara Y, Nagahori N, Amano M, Hinou H, Nishimura S.
    Glycoblotting-assisted O-glycomics: ammonium carbamate allows for highly efficient o-glycan release from glycoproteins.
    Anal Chem., 82, 10021-10029 (2010)
    PMID: 21077635

  39. Amano M, Nishimura S.
    Large-scale glycomics for discovering cancer-associated N-glycans by integrating glycoblotting and mass spectrometry.
    Methods Enzymol., 478, 109-25 (2010)
    PMID: 20816476

  40. Kurogochi M, Matsushista T, Amano M, Furukawa J, Shinohara Y, Aoshima M, Nishimura S.
    Sialic acid-focused quantitative mouse serum glycoproteomics by multiple reaction monitoring assay.
    Mol Cell Proteomics., 9, 2354-2368 (2010)
    PMID: 20571061

  41. Inagaki M, Nakaya S, Nohara D, Yabe T, Kanamaru Y, Suzuki T.
    The multiplicity of N-glycan structures of bovine milk 18 kda lactophorin (milk GlyCAM-1).
    Biosci Biotechnol Biochem., 74, 447-450 (2010)
    PMID: 20139591

  42. Amano M, Yamaguchi M, Takegawa Y, Yamashita T, Terashima M, Furukawa J, Miura Y, Shinohara Y, Iwasaki N, Minami A, Nishimura S.
    Threshold in stage-specific embryonic glycotypes uncovered by a full portrait of dynamic N-glycan expression during cell differentiation.
    Mol Cell Proteomics., 9, 523-537 (2010)
    PMID: 20008832

  43. Matsumoto K, Shimizu C, Arao T, Andoh M, Katsumata N, Kohno T, Yonemori K, Koizumi F, Yokote H, Aogi K, Tamura K, Nishio K, Fujiwara Y.
    Identification of predictive biomarkers for response to trastuzumab using plasma FUCA activity and N-glycan identified by MALDI-TOF-MS.
    J Proteome Res., 8, 457-462 (2009)
    PMID: 19140672

  44. Nagahori N, Abe M, Nishimura S.
    Structural and functional glycosphingolipidomics by glycoblotting with an aminooxy-functionalized gold nanoparticle.
    Biochemistry., 48, 583-594 (2009)
    PMID: 19117481

  45. Uematsu R, Shinohara Y, Nakagawa H, Kurogochi M, Furukawa J, Miura Y, Akiyama M, Shimizu H, Nishimura S.
    Glycosylation specific for adhesion molecules in epidermis and its receptor revealed by glycoform-focused reverse genomics.
    Mol Cell Proteomics., 8, 232-244 (2009)
    PMID: 18824476

  46. Furukawa J-i., Shinohara Y., Kuramoto H., Miura Y., Shimaoka H., Kurogochi M., Nakano M., Nishimura S.-I.
    Comprehensive approach to structural and functional glycomics based on chemoselective glycoblotting and sequential tag conversion
    Anal. Chem., 80, 1094-1101 (2008)
    PMID: 18205388

  47. Miura Y., Hato M., Shinohara Y., Kuramoto H., Furukawa J.-i., Kurogochi M., Shimaoka H., Tada M., Nakanishi K., Ozaki M., Todo S., and Nishimura S.-I.
    BlotGlycoABCTM: An integrated glycoblotting technique for rapid and large-scale clinical glycomics.
    Mol. Cell. Proteomics, 7, 370-377 (2008)
    PMID: 17986439

  48. Kurogochi M, Amano M, Fumoto M, Takimoto A, Kondo H, Nishimura S.
    Reverse glycoblotting allows rapid-enrichment glycoproteomics of biopharmaceuticals and disease-related biomarkers.
    Angew Chem Int Ed Engl., 46, 8808-8813 (2007)
    PMID: 17943939

  49. Fujimura T., Shinohara Y., Tissot B., Pang P.-C., Kurogochi M., Saito S., Arai Y., Sadilek M., Murayama K., Dell A., Nishimura S.-I., Hakomori S.
    Glycosylation status of haptoglobin in sera of patients with prostate cancer vs. benign prostate disease or normal subjects.
    Intl. J. Cancer , 122, 39-49 (2008)
    PMID: 17803183

  50. Kita Y., Miura Y., Furukawa J., Nakano M., Shinohara Y., Ohno M., Takimoto A., and Nishimura S.-I.
    Quantitative glycomics of human whole serum glycoproteins based on the standardized protocol for liberating N-glycans.
    Mol. Cell. Proteomics, 6, 1437-1445 (2007)
    PMID: 17522412

  51. Miura Y., Shinohara Y., Furukawa J., Nagahori N., and Nishimura S.-I.
    Rapid and Simple Solid-Phase Esterification of Sialic Acid Residues for Quantitative Glycomics by Mass Spectrometry.
    Chem. Eur. J. , 13, 4797-4804 (2007)
    PMID: 17372994

  52. Shimaoka H., Kuramoto H., Furukawa J., Miura Y., Kurogochi M., Kita Y., Hinou H., Shinohara Y., and Nishimura S.-I.
    One-Pot Solid-Phase Glycoblotting and Probing by Transoximization for High-Throughput Glycomics and Glycoproteomics.
    Chem. Eur. J. , 13 , 1664-1673 (2007)
    PMID: 17225232

  53. Onodera T, Niikura K, Iwasaki N, Nagahori N, Shimaoka H, Kamitani R, Majima T, Minami A, Nishimura S.
    Specific cell behavior of human fibroblast onto carbohydrate surface detected by glycoblotting films.
    Biomacromolecules., 7, 2949-2955 (2006)
    PMID: 17096518

  54. Hato M., Nakagawa H., Kurogochi M., Akama T. O., Marth J. D, Fukuda M. N., and Nishimura S.-I.
    Unusual N-Glycan Structures in α-Mannosidase II/IIx Double Null Embryos Identified by a Systematic Glycomics Approach Based on Two-dimensional LC Mapping and Matrix-dependent Selective Fragmentation Method in MALDI-TOF/TOF Mass Spectrometry
    Mol. Cell. Proteomics , 5 , 2146-2157 (2006)
    PMID: 16899540

  55. Uematsu R., Furukawa J., Nakagawa H., Shinohara H., Deguchi K., Monde K., and Nishimura S.-I.
    High throughput quantitative glycomics and glycoform-focused proteomics of murine dermis and epidermis.
    Mol. Cell. Proteomics , 4 , 1977-1989 (2005)
    PMID: 16170054

  56. Matsubara N, Oiwa K, Hohsaka T, Sadamoto R, Niikura K, Fukuhara N, Takimoto A, Kondo H, Nishimura S.
    Molecular design of glycoprotein mimetics: glycoblotting by engineered proteins with an oxylamino-functionalized amino acid residue.
    Chemistry.,11, 6974-6981 (2005)
    PMID: 16144022

  57. Niikura K., Kamitani R., Kurogochi M., Uematsu R., Shinohara Y., Nakagawa H., Deguchi K., Monde K., Kondo H., Nishimura S.-I.
    Versatile Glycoblotting Nanoparticles for High Throughput Protein Glycomics.
    Chem. Eur. J. , 11 , 3825-3835 (2005)
    PMID: 15827987
  58. Nishimura S.-I. , Niikura K., Kurogochi M., Matsushita T., Fumoto M., Hinou H., Kamitani R., Nakagawa H., Deguchi K., Miura N., Monde K., Kondo H.
    High-Throughput Protein Glycomics: Combined Use of Chemoselective Glycoblotting and MALDI-TOF/TOF Mass Spectrometry.
    Angew. Chem. Int. Ed. , 44 , 91-96 (2005)
    PMID: 15599899

和文

  1. 天野麻穂, 三浦嘉晃, 西村紳一郎
    グライコブロッティング法が可能にした大規模糖鎖解析
    生化学,83(1),5-12(2011)

  2. 西村紳一郎
    全自動糖鎖解析システムの開発-臨床検査バイオマーカーとしての糖鎖の可能性-
    医療と検査機器・試薬,34(1),53-60(2011)

  3. 天野 麻穂 , 西村 紳一郎
    高速化が進む糖鎖解析 - 糖鎖構造研究を加速する新しい解析法:グライコブロッティング法 -
    現代化学 , 東京化学同人 , 435 , 55-61 (2007)

  4. 西村 紳一郎
    Glycoblotting 法:高速糖鎖エンリッチ技術を用いる疾患グライコミクスの展開
    先端メディカル分子計測技術 第 6 回 JST-SENTAN シンポジウム 講演要旨集 , 32-35 (2006)

  5. 西村 紳一郎
    糖鎖を開始点とするリバースゲノミクス (GFRG) を目指して
    臨床化学 , 日本臨床化学会 , 34 , 297-308 (2005)